Establishment of Methylation-Specific PCR for the Mouse p53 Gene
نویسندگان
چکیده
منابع مشابه
Establishment of Methylation-Specific PCR for the Mouse p53 Gene
Methylation-specific PCR (MSP) of the mouse p53 gene has not yet been reported. We searched the CpG islands, sequenced the bisulfited DNA, and designed PCR primers for methylation and unmethylation sites. DNA from a young mouse produced a strong PCR product with the unmethylated primer and a weaker band with the methylated primer. DNA from an old mouse produced bands of similar intensities with...
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Gastric Cancer (GC) is the second most common cancer in the world and a leading cause of cancer-related mortality. Methylation of promoter CpG islands (CGIs) belonging to tumor suppressor genes causes transcriptional silencing of their corresponding genes leading to carcinogenesis and other disorders. Adenomatous Polyposis Coli (APC) a tumor suppressor gene is inactivated by methylation of prom...
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ژورنال
عنوان ژورنال: Molecular Biology International
سال: 2011
ISSN: 2090-2190
DOI: 10.4061/2011/938435